MiR-93 suppresses cell proliferation and invasion by targeting ZNF322 in human hepatocellular carcinoma

Introduction This study aimed to investigate the biological role of microRNA 93 (miR-93), a novel tumor-related miRNA, in human hepatocellular carcinoma (HCC) and elucidate potential molecular mechanisms involved. Material methods Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted determine expression miR-93 HCC tissues cell lines. The log-rank test Kaplan-Meier survival analysis were performed evaluate relationship between overall survival. MTT assay, colony formation Transwell migration invasion assays carried out exam proliferation, formation, invasion, respectively. Murine xenograft models established effect on tumor growth vivo. TargetScan online software applied predict target miR-93. Luciferase reporter used validate direct binding its putative target. Results Here we found that significantly down-regulated Patients with decreased had shorter Functional investigations demonstrated over-expression suppressed weakened clonogenic ability, slowed down invasion; whereas depletion facilitated invasion. MiR-93 retarded assay rescue revealed zinc finger protein 322 (ZNF322) mediated inhibitory effects proliferation motility. Conclusions Our data may provide some evidence for miR-93/ZNF322 axis candidate therapeutic HCC.